The easyCyte line of flow cytometers offer:
|Up to 3 lasers and 14 parameters on a benchtop instrument||High degree of flexibility|
|Innovative microcapillary fluidics design||Eliminates sheath fluid and reduces waste|
|Microcapillary technology and a positive displacement syringe pump||Direct absolute counting with industry-leading precision|
|Intuitive software interface||Simplified assessment of results|
|Detection limits of 0.2 to 60 µm||Evaluation of a broad variety of samples|
|Single sample and high-throughput options||Walkaway acquisition|
How Guava® easyCyte™ Systems Work
Single, dual, or triple excitation laser configurations provide up to 14 simultaneous detection parameters, including 12 fluorescent colors plus forward and side scatter for size and granularity determination. For applications requiring exceptional sensitivity, a high-power blue laser (HPL) is offered in both single-loader and high-throughput (HT) formats.
Accurate Cell Concentrations
Guava easyCyte systems combine microcapillary flow with a precision pump to provide accurate and precise absolute counts—without the use of reference beads.
Using the ViaCount™ reagent and module allows for the determination of absolute counts for live, apoptotic, and dead cells. Linear and accurate results can be seen across the multiple concentrations and on a variety of cell and sample types.
Consistent Sample Results Across an Entire Plate With Sample Mixing
To ensure that cells stay in suspension during sampling, a rotating, variable-speed mixing paddle with auto-cleaning is used between each well, eliminating the need for manual resuspension after loading the plate.
Guava easyCheck™ beads were diluted at 1.0 x 106 cells/mL and placed into all wells of a 96-well plate. The plate was then spun at 150 x g for 5 minutes to mimic settling. Each well was acquired and concentrations were determined. Results show the concentrations for each well (solid line) as well as +/- 10% values (dotted lines).
The mixing feature virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats. Data shown below confirms that carryover is minimized with this format.
|Plate 1||Plate 2||Plate 3||Plate 4||Plate 5|
|Carryover||0.15% ±||0.17% ±||0.21% ±||0.23% ±||0.22% ±|
InCyte™ Software Heat Mapping Feature
Heat map results output from InCyte Software showing four-parameter data for cell health impact of 80 compounds on Jurkat cells. Each well is mapped to a four-quadrant circle with color intensity reflecting the percentages of cell population positive for loss of mitochondrial membrane potential, Annexin V staining, caspase activation, and cell death.
- Application Note: Cytek® Guava® easyCyte™ and Cytek® Guava® Muse® Solutions for Cell and Gene Therapy
- Application Note: Plate-based Drug and Toxin Screening Using Multiplexed, Benchtop Flow Cytometry
- Cytek® Guava® easyCyte™ HT Flow Cytometer Overview
- Cytek® Guava® easyCyte™ SL Flow Cytometer Overview
|System Maintenance Reagents|
|Product||Instrument Type||Part Number|
|Guava® Instrument Cleaning Fluid (ICF)||All Guava® Products||4200-0140|
|Guava® Check Kit||easyCyte™, PCA, and easyCyte™ Plus||4500-0020|
|Guava® easyCheck™ Kit (100 tests)||easyCyte™ & easyCyte™ HT Series||4500-0025|
|Kits and Assays|
|Guava® SARS-CoV-2 Multi-Antigen Antibody Kit||Protein Detection||FCPA100101|
|Guava® ViaCount™ Reagent||Cell Health||
4000-0040 (40 mL)
4000-0041 (240 mL)
|Guava® ViaCount™ Flex Reagent (100 tests)||Cell Health||
4500-0110 (100 tests)
4700-0060 (500 tests)
|Guava® ViaCount™ Cell Dispersal Reagent||Cell Health||4700-0050|
|Guava® MitoDamage Kit||Cell Health||FCCH100106|
|Guava® Express 7-AAD Reagent||Cell Health||4000-0061|
|Guava® DNA Damage Histone H2A.X Dual Detection Kit||Cell Health||FCCS025153|
|Guava® Histone H2A.X Dual Detection Kit||Cell Pathways||FCCS100182|
|Guava® Autophagy LC3 Antibody-Based Detection Kit||Cell Health, Cell Pathways, Autophagy||FCCH100171|
|Guava® Annexin Red Kit||Cell Health, Early Apoptosis||FCCH100108|
|Guava® Nexin® Reagent Kit||Cell Health, Early Apoptosis||
4500-0450 (100 Tests)
4500-0455 (500 Tests)
|Guava® Cell Cycle Reagent Kit||Cell Health, Cell Cycle||4500-0220|
For Research Use Only. Not for use in diagnostic or therapeutic procedures.