Accurate Cell Concentrations

Guava easyCyte systems combine microcapillary flow with a precision pump to provide accurate and precise absolute counts—without the use of reference beads.

​Using the ViaCount™ reagent and module allows for the determination of absolute counts for live, apoptotic, and dead cells. Linear and accurate results can be seen across the multiple concentrations and on a variety of cell and sample types.

Consistent Sample Results Across an Entire Plate With Sample Mixing

To ensure that cells stay in suspension during sampling, a rotating, variable-speed mixing paddle with auto-cleaning is used between each well, eliminating the need for manual resuspension after loading the plate.

Guava easyCheck^™ beads were diluted at 1.0 x 10⁶ cells/mL and placed into all wells of a 96-well plate. The plate was then spun at 150 x g for 5 minutes to mimic settling. Each well was acquired and concentrations were determined. Results show the concentrations for each well (solid line) as well as +/- 10% values (dotted lines).

The mixing feature virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats. Data shown below confirms that carryover is minimized with this format.

InCyte^™ Software Heat Mapping Feature

Heat map results output from InCyte Software showing four-parameter data for cell health impact of 80 compounds on Jurkat cells. Each well is mapped to a four-quadrant circle with color intensity reflecting the percentages of cell population positive for loss of mitochondrial membrane potential, Annexin V staining, caspase activation, and cell death.