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Cytek Aurora™

Overview
Technology
Specifications
Software
Performance Data
Why Choose Aurora?

Overview


A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the next level of performance and flexibility.

With three lasers, two scattering channels, and up to 48 fluorescence channels, the Aurora suits every laboratory's needs, from simple to high-complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. Flat-Top beam profiles, combined with a uniquely designed fluidic system, translate to outstanding performance at high sample flow rates.

The end result is a system that delivers high quality data where rare and dim populations are easily resolved, regardless of assay complexity.

The new SpectroFlo™ software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running any application.

The Cytek team has reimagined every aspect of cytometry hardware and software to deliver an instrument that fulfills every researcher's needs.


Maximum Channels

50 channels of detection over the full emission spectra.


Maximum Colors

Up to 20 colors demonstrated including dyes with emission spectra in close proximity to each other.


Maximum Sensitivity

Sensitivity redefined using state-of-the-art optics and low-noise electronics.


Maximum Flexibility

No changing optical filters for any fluorochrome.
Use any commercially available dye excited by the lasers onboard.


Maximum Accessibility

A powerful, high value system that is accessible to a wide range of users.

Technology


Aurora's Revolutionary Technologies:

From Vision to Reality

The Aurora 3-laser system is capable of up to 50 detection channels (48 fluorescent channels, FSC, and violet laser SSC) and is empowered by revolutionary technologies, including:

  • Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) 16-channel semiconductor detector array for each laser, enabling more efficient spectrum capture for dyes emitting in the 400-900 nm range.
  • High bandwidth electronics design scalable beyond 50 channels.
  • Robust vacuum fluidics system enables ultimate flexibility in sample input formats.
  • Exceptional small particle detection is enabled by violet laser scatter, narrow beam height, and proprietary flat top laser design.

Specifications

Optics

Excitation Optics

Optical Platform

Aurora contains a fixed optical assembly configured with three spatially separated laser beams. Laser delays are automatically adjusted during instrument QC.

Lasers

405nm: 100mW, 488nm: 50mW, 640nm: 80mW

Beam geometry

Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.

Emission Optics

Emission Collection

Fused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.

Forward and Side Scatter Detection

FSC: high-performance semiconductor detector with 488nm bandpass filter.

Violet SSC: high-performance semiconductor detector with 405nm bandpass filter.

Fluorescence Detectors

Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) 16-channel semiconductor detector array per laser enabling more efficient spectrum capture for dyes emitting in the 400-900 nm range. No filter changes required for any fluorochrome excited by the 405nm, 488nm, and 640nm lasers.

Standard Optical Configuration

Violet detector module: 16 channels uneven spaced bandwidth from 420nm-830nm.

Blue detector module: 14 channels uneven spaced bandwidth from 500-890nm standard. Up to 16 channels available.

Red detector module: 8 channels uneven spaced bandwidth from 650-890nm standard. Up to 16 channels available.

Fluidics

Sample Flow Rates

Low: 15 µL/min, Medium: 30µL/min, High: 60µL/min

Fluidic Modes

Long clean, SIT flush, Purge filter, Degas flow cell, Clean flow cell

Manual Sample Input Formats

12x75mm polystyrene and polypropylene tubes

Standard Fluidic Reservoirs

4L fluid container set with level-sensing provided. Compatible with 20L sheath and waste cubitainers.

Plate Loader Option

96-well microtiter plate capability available in 2018.

Sample Dead Volume

5μl for 12x75mm tube

Performance

Fluorescence Sensitivity

FITC: 100 MEFL, PE: 30 MEFL, APC: 15 MEFL, Pacific Blue: 200 MEFL

* measurements based on an average from three systems and performed using SPHERO Rainbow Calibration Particle (RCP-30-5A) based on its peak emission channel.

Fluorescence Linearity

FITC R2 ≥0.995 / PE R2 ≥0.995

Forward and Side Scatter Sensitivity

Enables separation of fixed platelets from noise.

Forward and Side Scatter Resolution

Performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Side Scatter Resolution

Capable of resolving 0.2µm beads from noise.

Carryover

<0.1%

Data Acquisition Rate

35,000 events/s.

Software

SpectroFlo™ Software

Live unmixing during acquisition

Developed specifically to streamline assay setup, data acquisition, and file export.

Automated QC module

Auto-fluorescence extraction

Raw and Unmixed FCS 3.1 files

Electronics

Signal Processing

Digital signal processing with automatic window gate adjustment.

22-bit 6.5 log decades.

Threshold using any single parameter or combination of parameters.

Pulse Shape Parameters

Pulse Area and Height for every parameter.

Width for scatter parameters and one fluorescence parameter for each laser.

Workstation

Operating System

Windows® 10 Pro 64-bit

Processor

Intel® Core™ i7 processor, 3.0 GHz

RAM

16GB, 16000 MHz DDR4 SO-DIMM

Hard Drive

500GB SATA 3.0Gb/s

Video Processor

Intel® HD Graphics 530

Monitor

28” UHD

Installation Requirements

Dimensions (W x D x H)

Instrument Dimensions

54 x 52 x 52 cm

Instrument Weight

61 kg

Computer Dimensions

3.45 x 18.29 x 17.9 cm

Recommended Workspace

152.4 x 61 x 132 cm

Room Requirements

Power

100-240V, 50/60 Hz, 2A max

Heat Dissipation

500W with all solid-state lasers

Temperature

15–28°C

Humidity

20%-85% relative non-condensing

Air filtering

No excessive dust or smoke

Lighting

No special requirements

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Documents


Aurora™ Product Brochure

Click below to download the complete product brochure for the Aurora. It contains everything you need to know about this revolutionary product.

Download PDF

Software


SpectroFlo Software Guided Workflows

The new SpectroFlo software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running any application.

Step 1: Create Your Experiment

Define your experiment, fluorochromes, labels, tubes, worksheets, and stopping criteria in this guided workflow.

Step 2: Acquire Your Tubes

Load and acquire your samples.

Step 3: Unmix Your Data

Use the unmixing algorithm to visualize your data (in real time or post acquisition).

Performance Data


Expanding a 7-Color Panel to 20 Colors... Is it Possible?

The optical design combined with the unmixing capability in SpectroFlo software allows you to easily expand panels. The 3 laser configuration provides outstanding multiparametric data for a wide array of applications.

Expanding to 20 Colors: A 20-color panel preserving the original 7-color assay is summarized in the table below. Reagents shared by the 7-color and 20-color panels are shown in bold.

Specificity Fluorochrome Specificity Fluorochrome Specificity Fluorochrome
CD45RA Alexa Fluor 488 CD56 APC IgD BV421
CD3 Alexa Fluor 532 CD27 Alexa Fluor 700 CD16 eFluor 450
CCR5 PE HLA DR APC/Fire 750 CD4 BV510
CD127 PE/Dazzle 594 CD14 Pacific Orange
CD11c PE-Cy5 CD20 BV570
CD123 PerCP-Cy5.5 CD8 BV605
PD-1 PerCP-eFluor710 CD25 BV650
CD38 PE-Cy7 CD11b BV711
CCR7 BV785

The 20-Color Panel Includes Many Highly Overlapping Dyes:

(Click to Enlarge)

Fluorescent Proteins and Challenging Dye Combinations

The detection of some fluorescent protein or fluorochrome combinations by conventional flow cytometry presents a challenge due to high amounts of spectral overlap (figures 1, 4). The Aurora addresses this challenge by using differences in full emission spectra signatures across all lasers to clearly resolve these combinations, even if the populations are co-expressed (figures 2, 3, 5 and 6).

(Click to Enlarge)

Why Choose Aurora?


Cytek Aurora Competitor Top 13 Color Cytometer Competitor 28 Color Cytometer Competitor 30+ Color Cytometer Competitor Spectral Cytometer
Maximum number of detectors per laser 16 5 10 10 32
Spatially separated lasers Yes Yes Yes Yes No
20-color assay sensitivity Excellent N/A Average Average Sub-optimal
Supported fluorescent tags All existing dyes Limited by optical filters provided Limited by optical filters provided Limited by optical filters provided Limited: red and violet lasers are co-linear
Detection emission wavelength range 400-900nm 400-800nm 400-800nm 400-800nm 500-800nm, 430nm, 460nm
Special fluorochromes needed for 20 color assay None N/A None, but limited fluorochrome choices Yes, but limited to exclusive fluorochromes None, but limited fluorochrome choices
Ability to test new dyes excited by supported lasers Yes Requires new filters Requires new filters Requires new filters Yes
Instrument setup to optimize sensitivity Automatic Manual Manual Manual Manual
Unmixing capability for overlapping dyes Yes No No No Yes
Able to remove cell autofluorescence Yes No No No Yes
Footprint Small Very Small Medium Large Medium

Stain Index Comparison

(Click to Enlarge)