A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the next level of performance and flexibility.
With three lasers, three scattering channels, and up to 48 fluorescence channels, the Aurora suits every laboratory's needs, from simple to high-complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. Flat-Top beam profiles, combined with a uniquely designed fluidic system, translate to outstanding performance at high sample flow rates.
The end result is a system that delivers high quality data where rare and dim populations are easily resolved, regardless of assay complexity.
The new SpectroFlo™ software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running any application.
The Cytek team has reimagined every aspect of cytometry hardware and software to deliver an instrument that fulfills every researcher's needs.
51 channels of detection over the full emission spectra.
>20 colors including dyes with emission spectra in close proximity to each other.
Sensitivity redefined using state-of-the-art optics and low-noise electronics.
No changing optical filters for any fluorochrome.
Use any commercially available dye excited by the lasers onboard.
A powerful, high value system that is accessible to a wide range of users.
Aurora's Revolutionary Technologies:
From Vision to Reality
The Aurora 3-laser system is capable of up to 51 detection channels (48 fluorescent channels, FSC, violet laser SSC, and blue laser SSC) and is empowered by revolutionary technologies, including:
- Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) 16-channel semiconductor detector array for each laser, enabling more efficient spectrum capture for dyes emitting in the 400-900 nm range.
- High bandwidth electronics design scalable beyond 51 channels.
- Robust vacuum fluidics system enables ultimate flexibility in sample input formats.
- Exceptional small particle detection is enabled by violet laser scatter, narrow beam height, and proprietary flat top laser design.
Aurora contains a fixed optical assembly configured with three spatially separated laser beams. Laser delays are automatically adjusted during instrument QC.
405nm: 100mW, 488nm: 50mW, 640nm: 50mW
Flat-Top laser beam profile with narrow vertical beam height optimized for small particle detection.
Fused silica cuvette coupled to high NA lens for optimum collection efficiency to optical fibers.
Forward and Side Scatter Detection
FSC: high-performance semiconductor detector with 488nm bandpass filter.
Violet SSC: high-performance semiconductor detector with 405nm bandpass filter.
Blue SSC: high-performance semiconductor detector with 488nm bandpass filter.
Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) 16-channel semiconductor detector array per laser enabling more efficient spectrum capture for dyes emitting in the 400-900 nm range. No filter changes required for any fluorochrome excited by the 405nm, 488nm, and 640nm lasers.
Standard Optical Configuration
Violet detector module: 16 channels uneven spaced bandwidth from 420nm-830nm.
Blue detector module: 14 channels uneven spaced bandwidth from 500-890nm standard. Up to 16 channels available.
Red detector module: 9 channels uneven spaced bandwidth from 650-890nm standard. Up to 16 channels available.
Sample Flow Rates
Low: 12 µL/min, Medium: 30µL/min, High: 60µL/min
Continuous flow rate from 10µL-60µL/min
Long clean, SIT flush, Purge filter, Degas flow cell, Clean flow cell
Manual Sample Input Formats
12x75mm polystyrene and polypropylene tubes
Standard Fluidic Reservoirs
4L fluid container set with level-sensing provided. Compatible with 20L sheath and waste cubitainers.
Plate Loader Option
96- or 384-well microtiter plate capability starting in December 2017.
Sample Dead Volume
5μl for 12x75mm tube
FITC: 100 MEFL, PE: 30 MEFL, APC: 15 MEFL, Pacific Blue: 200 MEFL
* measurements performed using SPHERO Rainbow Calibration Particle (RCP-30-5A) based on its single peak detection channel.
FITC R2 ≥0.995 / PE R2 ≥0.995
Forward and Side Scatter Sensitivity
Enables separation of fixed platelets from noise.
Forward and Side Scatter Resolution
Performance is optimized for resolving lymphocytes, monocytes, and granulocytes, as well as microparticles.
Side Scatter Resolution
Capable of resolving 0.2µm beads from noise.
Data Acquisition Rate
Live unmixing during acquisition
Developed specifically to streamline assay setup, data acquisition, and file export.
Automated QC module
48 unmixed parameters
Raw and Unmixed FCS 3.1 files
Digital signal processing with automatic window gate adjustment.
22-bit 6.5 log decades.
Threshold using any single parameter or combination of parameters.
Pulse Shape Parameters
Pulse Area and Height for every parameter.
Width for scatter parameters and one fluorescence parameter for each laser.
Windows® 10 Pro 64-bit
Intel® Core™ i7-6700T processor, 3.0 GHz
16GB, 16000 MHz DDR4 SO-DIMM
500GB SATA 3.0Gb/s
Intel® HD Graphics 530
Dimensions (W x D x H)
54 x 52 x 52 cm
3.45 x 18.29 x 17.9 cm
152.4 x 61 x 132 cm
100-240V, 50/60 Hz, 2A max
500W with all solid-state lasers
20%-85% relative non-condensing
No excessive dust or smoke
No special requirements
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
SpectroFlo Software Guided Workflows
The new SpectroFlo software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running any application.
Step 1: Create Your Experiment
Define your experiment, fluorochromes, labels, tubes, analysis worksheets, and stopping criteria in this guided workflow.
Expanding a 7-Color Panel to 20 Colors... Is it Possible?
The optical design combined with Aurora's unmixing capability allows you to easily expand panels. The 3 laser configuration provides outstanding multiparametric data for a wide array of applications.
Expanding to 20 Colors: A 20-color panel preserving the original 7-color assay is summarized in the table below. Reagents shared by the 7-color and 20-color panels are shown in bold.
|CD45RA||Alexa Fluor 488||CD56||APC||IgD||BV421|
|CD3||Alexa Fluor 532||CD27||Alexa Fluor 700||CD16||eFluor 450|
|CCR5||PE||HLA DR||APC/Fire 750||CD4||BV510|
|CD127||PE/Dazzle 594||CD14||Pacific Orange|
The 20-Color Panel Includes Many Highly Overlapping Dyes:
Fluorescent Proteins and Challenging Dye Combinations
The detection of some fluorescent protein or fluorochrome combinations by conventional flow cytometry presents a challenge due to high amounts of spectral overlap (figures 1, 4). The Aurora addresses this challenge by using differences in full emission spectra signatures across all lasers to clearly resolve these combinations, even if the populations are co-expressed (figures 2, 3, 5 and 6).
(Click to Enlarge)
Why Choose Aurora?
|Cytek Aurora||Competitor Top 13C Cytometer||Competitor 28C Cytometer||Competitor 30C+ Cytometer||Competitor Spectral Cytometer|
|Maximum number of detectors per laser||16||5||10||10||32|
|20-color assay sensitivity||Excellent||N/A||Average||Average||Sub-optimal|
|Supported fluorescent tags||All existing dyes||Limited by optical filters provided||Limited by optical filters provided||Limited by optical filters provided||Limited: red and violet lasers are co-linear|
|Detection emission wavelength range||400-900nm||400-800nm||400-800nm||400-800nm||500-800nm, 430nm, 460nm|
|Special fluorochromes needed for 20 color assay||None||N/A||None, but limited fluorochrome choices||Yes, but limited to exclusive fluorochromes||None, but limited fluorochrome choices|
|Ability to test new dyes excited by supported lasers||Yes||Requires new filters||Requires new filters||Requires new filters||Yes|
|Instrument setup to optimize sensitivity||Automatic||Manual||Manual||Manual||Manual|
|Unmixing capability for overlapping dyes||Yes||No||No||No||Yes|
|Able to remove cell autofluorescence||Yes||No||No||No||Yes|