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Time Delay Integration Technology

The Cytek® Amnis® family of flow cytometers utilize a charge coupled device (CCD) operated in time delay integration (TDI) mode. This mode allows image capture of moving objects with very high image quality and exceptional photonic sensitivity. In TDI mode, the photon charge is transferred down each pixel row in precise synchrony with the transit of the cell, allowing for increased light integration time. This effect is similar to physically panning a camera with the object. Each pixel row is then read off the bottom of the chip and is used to reconstruct the image of the cell for investigation using image analysis software tools.

Cytek® Amnis® Imaging Flow Cytometers

Flow Cytometer Imaging Flow Cytometers
Cytek® Amnis® CellStream® Cytek® Amnis® FlowSight® Cytek® Amnis® ImageStream®X MKII
Attribute Sensitive Visual Boundless
Lasers 1-7 1-4 1-6
Intensity Up to 22 Up to 10 Up to 10
Morphology 3 >1,000 >1,000
Magnification N/A 20x Up to 60x
Format Open Open / Pre-Optimized Kits Open / Pre-Optimized Kits
Flexibility Highest High Highest
Microscopy Applications Event Gallery Yes Yes
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ImageStream®X MKII Optical Layout

Optical layout of the ImageStream® imaging flow cytometer: Cells are fluorescently labeled in suspension and loaded through the system using a syringe pump. Samples are hydrodynamically focused in flow, and fluorescence is excited using multiple laser lines. The brightfield, SSC and fluorescent images are captured by a 20x, 40x or 60x imaging objective and transferred to the filter stack where each color is projected to a spatially discrete channel on the cameras. Up to 10 colors of fluorescence are collected in spatial registry, so each probe is measured for intensity, morphology and relative location to other probes.

CellStream® Optical Layout

Our patented time delay integration (TDI) and camera technology deliver sensitivity and expandability beyond what is possible with traditional flow cytometers:

  1. Up to 7 lasers are focused in discrete locations, aligned to four zones.
  2. Syringe Driven Fluidics: Hydrodynamically focused cells pass through the laser-illuminated region. Fluorochromes bound to the cells are excited and emit into the collection system. Fluorescence is collected and directed toward an intermediate image plane.
  3. A six-channel filter stack decomposes each of the four discrete vertical positions in the intermediate image plane into 22 separate channels of data.
  4. All 22 channels fit efficiently onto a charge-coupled device (CCD) array. The sensor contains multiple discrete collection fields using the same CCD as patented Amnis® technology.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.