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The Cytek Northern Lights flow cytometer, with its easy-to-use workflow, is a flexible and affordable full spectrum flow cytometry solution for everyday research applications. The Northern Lights instrument incorporates Cytek’s powerful Full Spectrum Profiling (FSP) technology in a one to three laser system, allowing for a wide array of new fluorochrome combinations without reconfiguring your system for each application. Whether you are doing basic assays such as cell proliferation, apoptosis analysis, or other cell health applications, or multicolor immunophenotyping panels, the Northern Lights system meets all of your flow cytometry needs.

SpectroFlo® software provides a user-friendly guided workflow from quality control to data analysis, incorporating tools to make running applications simpler.

Northern Lights™ Flow Cytometry Instruments
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Easy, Flexible, and Intuitive

Enhance efficiency with one configuration for simple assays to more advanced analyses, using any readily available fluorochrome and our intuitive software.
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Low Cost of Ownership
 

Designed to run more colors with fewer lasers for greater cost savings, expanded capabilities, and less setup time between experiments.
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Remarkable Sensitivity
 

High sensitivity with state of the art optics and low noise electronics resolves even the dimmest populations.
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High Value
 

Adaptable from a single laser for nine color detection, to a three laser system capable of detecting 24 colors, there is a Northern Lights configuration to fit your needs.

Testimonials and Publications

Investigating Sample Heterogeneity
Hear from Anna Brooks, senior research fellow and director of Auckland Cytometry at the University of Auckland, about her experiences using the Cytek Aurora and Cytek Northern Lights cytometers in her research.
Adapting to Multiple Users Needs
Bridget McLaughlin, co-director of the Flow Cytometry Shared Resource Lab at the University of California Davis, shares her experiences bringing a Cytek full spectrum flow cytometer to her multi-user shared instrument facility.

Service Plan Comparison

Maintaining your cytometry equipment does not have to be prohibitively expensive. Cytek's service through technical excellence offers lab managers cost effective service plans, without sacrificing quality assurance.
Service Contract Types
The following on-site service contract types are available through Cytek®:

Value

Includes
  • Parts
  • Travel
  • Labor
  • Phone Support
  • 1 Maintenance Visit/Year
  • $750 deductible per emergency service visit.

Premium

Includes
  • Parts
  • Travel
  • Labor
  • Phone Support
  • 1 Maintenance Visit/Year
  • Guaranteed Response Time

Preventative Maintenance

Includes
  • Phone Support
  • 2 Maintenance Visits/Year
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  •  
  •  
  • All other parts and services billed at prevailing rate.

Related Products

Counting and Viability

The Cytek Northern Lights full spectrum cell analyzer, along with the Guava® ViaCount reagent assay, offers precise and accurate counting and viability determination for many different cell types. The high sensitivity of the Northern Lights instrument offers excellent resolution of populations that may be missed on conventional systems. The workflow is easy and requires minimal effort to acquire counts and viability of cell populations.

Cytek Northern Lights Full Spectrum Flow Cytometer Counting and Viability

Live and dead Jurkat cells were mixed and stained with the Guava ViaCount reagent. Analysis was performed on the Northern Lights system. Percentages shown are the percent of live and dead cells in each sample.

Cytek Northern Lights Full Spectrum Flow Cytometer Apoptosis Analysis

Jurkat cells were induced into undergoing apoptosis with staurosporine treatment. The induced cells, and the live and dead cell controls, were analyzed on the Northern Lights system. The Guava Nexin® assay was used to determine each population. Clear distinction of each population was achieved as shown.

Apoptosis Analysis

Easily detect and measure cell populations progressing through the apoptotic pathway using the Northern Lights full spectrum flow cytometer and the Guava Nexin® assay. You can determine and differentiate between live, dead, and early apoptotic populations in a quick and simple way.

Cell Cycle Analysis

The Northern Lights instrument is a powerful tool for flow cytometry cell cycle analysis. Using the Northern Lights system, one can easily determine the discrete phases of the cell cycle with percent CVs well under 3%. Not only can you analyze cell cycle progression, but you can multiplex with phenotyping of important cell cycle protein markers. The beauty of Full Spectrum Profiling (FSP) technology allows for deeper insights into cell division and cell cycle analysis.

Cytek Northern Lights Full Spectrum Flow Cytometer Cell Cycle Analysis

The cell cycle inhibitor, nocodazole, was used to arrest cells in the G2/M phase of the cell cycle. Samples were treated, prepared with the Guava® Cell Cycle reagent, and data were acquired on the Northern Lights flow cytometer. FCS files were analyzed using ModFit LT software (Verity Software House).

Cytek Northern Lights Full Spectrum Flow Cytometer Autofluorescence Extraction
Autofluorescence Extraction

The Northern Light system’s implementation of full spectrum flow cytometry enables the use of autofluorescence extraction to further improve data clarity. Certain sample types, such as yeast and tumor samples, present the challenge of high autofluorescence. For these challenging applications involving highly autofluorescent particles, let the software's autofluorescence extraction tool bring new levels of resolution. SEE POSTER

Everyday Immunoprofiling

Full spectrum flow cytometry opens a wide range of new possibilities. The Northern Lights system allows scientists to run complex multicolor experiments with as few as one or two lasers. The unique optical system enables the use of dyes with highly overlapping peak emissions without sacrificing resolution, translating to more flexibility in dye choice.
9 Colors
21 Colors
9 Colors

The one laser (488 nm) Cytek® Northern Lights system can perform 9-color immunoprofiling assays with no sacrifice to data quality. In this 9-color blue laser excitable dyes panel, monocytes and several CD4 T cell and CD8 T cell subsets were easily identified.

Cytek Northern Lights Full Spectrum Data from Blue Excited Dyes
9-Colors Panel Dyes Emission Spectra
SEE DATASHEET
21 Colors

With 30 fluorescence detectors and only 2 lasers (405 and 488 nm), the Cytek® Northern Lights system enables multi-parametric data collection with few lasers to maintain. Cytek has developed a 21-color human immunophenotyping panel acquired from just a single tube, with outstanding resolution.

Blue Excited Dyes Emission Spectra
 
SEE DATASHEET

Class 1 Laser Product.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.