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The Cytek® Amnis® FlowSight® imaging flow cytometer is a high value small footprint system that captures fluorescence, brightfield and SSC images in 12 channels at up to 4000 objects per second. The proprietary TDI CCD camera yields high photonic sensitivity with high image quality and when combined with Amnis image analysis software suite enables a broad range of applications. The FlowSight imaging flow cytometer is powerful enough for the core lab, but sized and priced for any lab. Whether in a base configuration or fully-optioned, the FlowSight instrument sets a new standard of value.

Intuitive: Easy to Use, With Imagery for Every Cell

The FlowSight instrument operates like a conventional flow cytometer, but also provides imagery of every cell. Its powerful and intuitive analysis software seamlessly links quantitative data to imagery:

  • Click on a dot in any plot to see the corresponding cell imagery
  • Click on a bin in any histogram to view all the cells in that bin
  • Draw gates on dot plots and view the resulting populations to validate results

High Value

Field upgradable to 4 lasers, a 96 well plate autosampler and access to the full Amnis® image analysis software suite FlowSight is big data, in a small package.

Remarkable Sensitivity

Low noise TDI CCD cameras provide exceptional photonic sensitivity.

Easy, Flexible, and Intuitive

With a small footprint, fast fluidics, and 8 easy to follow workflows, the FlowSight is ready for any lab​.
Performance Characteristics 20x Magnification
Numeric Aperture 0.6
Pixel Size 1.0 x 1.0 µm
Field of View 60 x 256 µm
Imaging Rate 4,000 Obj/Sec

Sample Characteristics

  • Volume: 20-200 ul
  • Utilization Efficiency: up to 95%

Physical Characteristics

  • 18" W x 18" H x 25" D (457 mm x 465 mm, 635 mm)
  • 135 lbs. (61 kg)

Automated Instrument Operations

  • Start up and shut down
  • Sample load, acquisition, compensation, batch analysis
  • Laser alignment, focus, calibration, and test


  • Excitation: Standard 488 nm; Optional 405 nm, 561 nm, and 642 nm
  • Side Scatter: 785 nm standard
  • Brightfield: Customizable in any channel

Operational Requirements

  • 450 W, 100-240 VAC, 50/60 Hz
  • No external air or water required

FlowSight® Kits

Product Category Part Number
Amnis® NFkB Translocation Kit Cell Pathway ACS10000
Amnis® Protein Aggregation & Silicone Oil Detection Kit Drug Discovery ACS10001
Amnis® FlowSight® System Calibration Reagent, FS400300 Calibration CN-0441-01


The Flowsight® system offers field upgradeable options for a configuration customized to your experiments.

  • Autosampler 96-well plate
  • Up to 4 lasers 405 nm, 488 nm, 561 nm, 642 nm

Software Options

IDEAS® Image Analysis Software: IDEAS software is powerful and easy to use, allowing users to create publication ready figures of their ImageStream® and FlowSight® data. The software combines familiar tools from cytometry and microscopy software including dot plots, histograms, stats tables, image gallery display optimization and more.

  • Wizards for easy-to-follow standard workflows such as Apoptosis, Cell Cycle-Mitosis, Co-localization, Internalization, Nuclear Localization, Shape Change, Spot Counting and the Feature Finder wizard that allows researchers to find the best feature for their unique experiment
  • Compensation wizard that applies spectral crosstalk correction for each pixel in the file based on single color controls
  • 86 features per channel measuring both intensity and morphology information
  • 22 function masks that identify sub-cellular components and refine the region of interest
  • Customizable statistics tables
  • Templates that facilitate repeat experiments and standardize analysis for all samples
  • Batch processing for automated compensation and processing of each sample

Machine Learning (ML) Module: ML is a plug in for the IDEAS software that enables generation of a novel feature specifically tailored to identify the desired cell morphology. It greatly simplifies analysis by allowing researchers to hand tag truth populations and use those images to automatically create a unique classifier that will find like images.

Compliance-Enabled Software Solutions: INSPIRE data acquisition software and IDEAS® data analysis software are available in 21 CFR part 11 enabled versions that allow users to meet requirements for regulated settings.

Amnis® AI Image Analysis Software: Amnis AI software represents the next generation of image analysis software. This stand-alone software package leverages computer aided hand tagging, clustering with object map plots, and generation of a novel experimental model using deep learning with convolutional neural networks (CNN) and Random Forest algorithms to classify any cell morphology. The software allows scientists to visually categorize multiple cell classes and train the computer to identify those populations in unknown samples producing easy to understand results including a confusion matrix table, probability index, precision, recall and FI statistics.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.