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GENOMICS WORKFLOW EXAMPLE

Leveraging Sequencing Data to Design Fast Immunoprofiling Flow Cytometry Assays

In the search for differentiable cell characteristics that correlate to disease states and symptom severity, researchers collect, sequence, and compare samples from patients and healthy individuals to search for clues. While gene sequencing technologies provide rich datasets, the results can take several days to obtain and review.

Translating findings from the sequencing data to a faster technology like the full spectrum flow cytometer Cytek Aurora, where the time to result is a few hours or less, enables clinicians to get the information they need to help their patients sooner.

Learn more about Cytek Aurora.

GENOMICS WORKFLOW EXAMPLE

Isolating Single Cells for Downstream Genetics Research

In heterogeneous cell suspensions, researchers can study gene expression of the entire sample to obtain average gene expression characteristics.

To go deeper and learn more about gene expression for the individual cell types, researchers must find ways to isolate single cells. Isolating living cells to extract their genetic information is a challenging task, and it’s crucial in understanding cellular relationships and identifying potential targets to treat a disease. It requires powerful computing and electronics capable of deciding which cells to collect in a matter of nanoseconds, gentle handling of the cells so they remain functional and intact, and precise targeting to deposit each cell into a small collection well of a microtiter plate.

Technological advances in flow cytometry sorters such as the Cytek Aurora CS have made this feat possible, enabling researchers to take their sorted cells of interest to downstream genetics tools, and connect downstream gene expression results with surface protein expression for powerful insights.

Learn more about Cytek Aurora CS.

The Cytek Aurora CS is used in step 2 of this workflow to accurately isolate the desired cell type.