PRODUCT BROCHURE
The Cytek Orion reagent cocktail preparation system enables researchers to automate the preparation of antibody cocktails for flow cytometry. The Cytek Orion system delivers walk-away automation to empower researchers to concoct up to 60 antibodies with speed, accuracy, and consistency while reducing hands-on time.
Key features of the Cytek Orion system:
- Automated robotic pipetting: Reduce hands-on time with robust and reliable cocktail preparation that utilizes a robotic interface
- Flexible reagent use: Use a variety of reagent tubes with volumes of 0.5 mL, 2.0 mL, and 5.0 mL, and the aspirator can add 6 μL to 200 μL of reagent to each cocktail
- Customizable cocktails: Prepare pre-defined or customize your own cocktail recipes that can be saved for reuse
- Optimal reagent protection: Built-in cooling and light shielding preserve reagent integrity
Cytek Orion Reagent Cocktail Preparation System Workflow
The workflow to set up the Cytek Orion system for a pre-defined or new cocktail recipe is designed to be straightforward and simple. The Cytek Orion software, SpectroPrep™, follows the conventions of Cytek SpectroFlo® software for familiarity and ease of use. A wizard guides users through adding reagents to the library, creating reagent deck settings, and creating cocktail recipes that can be saved and reused. This information can also be uploaded from a CSV file format.
During cocktail preparation, the software will show you the status of the worklist with indicators of tip usage and steps completed or in process.
| Feature | Description | Specification |
|---|---|---|
| System Performance | Carryover | 0% |
| Accuracy |
6-9 μL, ±8% by volume 10-50 μL, ±5.5% by volume 51-200 μL, ±5% by volume |
|
| Precision |
6-9 μL CV<5% 10-50 μL CV<5% 51-200 μL CV<1.5% |
|
| Throughput | Average of <70 seconds/tip, withdraw 50 μL | |
| Sample and Reagent Volumes | Antibodies | Cytek standard 0.5 mL and 2.0 mL reagent vials and supports commonly used 5 mL reagent vial formats |
| Buffers | 10 mL bottle | |
| Sample Tube Capacity | - | 5 mL, 15 mL, and 50 mL tubes |
| Dead Volume | - | <15 µL for 0.5 mL Cytek antibody vials <25 µL for 2.0 mL Cytek antibody vials Average 100 µL for 5 mL antibody vials Average 110 µL for 10 mL buffer bottle |
| Reagent Blocks | - | 60 positions to fit Cytek standard reagent vials and other commonly used reagent vial formats |
| Weight | - | 70 kg |
| Dimensions | W x D x H (mm) |
660 mm x 555 mm x 590 mm 1080 mm x 555 mm x 590 mm with screen expanded |
| Power Source | - | 110 V - 240 V AC |
| Environment | Operating Temperature | 15°C to 28°C |
| Operating Relative Humidity | 20% to 80% non-condensing | |
| Noise Level | 65 dBA |
| PN | Description |
|---|---|
| N7-00120 | Cytek Orion Reagent Cocktail Preparation System |
| N0-10172 | Cytek Orion Nature, Low Retention 200 μL Tips |
| N0-10213 | Cytek Orion Nature, Pre-Sterilized, Low Retention 200 μL Tips |
| N0-10214 | Cytek Orion Nature, Filtered, Pre-Sterilized, Low Retention 200 μL Tips |
| N0-10211 | Cytek Orion Nature, Low Retention 50 μL Tips |
| N0-10353 | Cytek Orion Nature, Low Retention, Pre-Sterilized 50 μL Tips |