TDS | SDS

The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

Name

violetFluor™ 500 Anti-Human CD8a (RPA-T8)

Cat. No.

85-0088

Alternative Names

CD8 alpha, leu-2a

Gene ID

925

Clone

RPA-T8

Isotype

Mouse IgG1, kappa

Reactivity

Human

Cross Reactivity

Chimpanzee, Baboon, Cynomolgus, Rhesus, Sooty Mangabey

Format

violetFluor™ 500

Application

Flow Cytometry

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