Recommended replacement for the discontinued product, Cytek^® FSP^® CompBeads, SKU B7-10011

Cytek^® Universal CompBeads are for use in flow cytometry. Fluorochrome-conjugated antibody binds the spherical particles for use as single-color compensation controls. They bind with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies.

PRODUCT DETAILS

Reactivity: Mouse, Rat, Hamster, Human, Rabbit IgG and IgM

Volume Per Test: 1 drop / test. Each drop contains approximately 1x10⁵ beads.

Application: Flow cytometry

Storage: 2-8°C. Do not freeze.

Data Sheets: TDS | SDS

APPLICATION

Each drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed. This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.

RECOMMENDED USAGE

1. Label tubes: Label a tube for each antibody conjugate to be used in the experiment.
2. Mix beads: Mix the CompBeads by vigorously inverting at least 10 times or by pulse vortexing.
3. Add beads: Add 1 drop of CompBeads to each tube (there is no need to add staining buffer).
4. Add Antibody Conjugate: Add 1 test antibody conjugate to each tube.

   Note: A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. If high background is observed on the negative bead population, use 0.125 µg or less antibody. It is not necessary to use the antibody at its optimal concentration. For most antibodies, appropriate compensation or unmixing values result when 0.03–1.0 µg of antibody is used in the test.

5. Mix the tube well by flicking or pulse-vortexing.
6. Incubate: Incubate at 2–8°C for 15–30 minutes in the dark.
7. Wash: Add 2–3 mL of Flow Cytometry Staining Buffer to each tube, then centrifuge at 400–600 × g for 3–5 minutes.
8. Decant the supernatant, then add 0.2–0.4 mL of Flow Cytometry Staining Buffer to each tube.
9. Mix by flicking or pulse-vortexing before acquisition.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.