The mouse monoclonal antibody clone SK3 binds to human CD4. Human CD4 is a monomeric, approximately 59 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It comprises four extracellular immunoglobulin (Ig)-like domains (D1-D4), with the D1 domain specifically binding to the β2 domain of Major Histocompatibility Complex (MHC) class II molecules on antigen-presenting cells (APCs). This interaction is a co-receptor alongside the T Cell Receptor (TCR) during antigen recognition, facilitating TCR signaling and subsequent T cell activation. The cytoplasmic tail of CD4 is associated with the tyrosine kinase Lck, which is crucial for initiating the signaling cascade that leads to T cell activation. CD4 is predominantly expressed on thymocytes and subsets of mature T cells, including T helper cells (Th17) and regulatory T cells (Treg), as well as on dendritic cells, monocytes, and macrophages. It plays a pivotal role in immune response modulation by aiding the activation of other immune cells, such as B cells and cytotoxic T lymphocytes, through cytokine release. Additionally, CD4 serves as a primary receptor for the Human Immunodeficiency Virus type 1 (HIV-1), which binds to CD4 and co-receptors CCR5 or CXCR4 to gain entry into T cells, leading to the depletion of these crucial immune cells and progression to Acquired Immunodeficiency Syndrome (AIDS). The SK3 antibody does not block the binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

PRODUCT DETAILS

Clone: SK3

Specificity: CD4

Reactivity: Human

Format: cFluor^® YG600

Isotype: IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® YG600 is equivalent to CF^®575 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.