The mouse monoclonal antibody clone 3G8 binds to human IgG receptor III (FcγRIII) that are in two forms: CD16a (FcγRIIIA) and CD16b (FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kDa polypeptide-anchored transmembrane protein and a 48 kDa GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages, while CD16b is expressed on neutrophils. CD16a also plays a crucial role in antibody-dependent cellular cytotoxicity (ADCC) by NK cells.

PRODUCT DETAILS

Clone: 3G8

Specificity: CD16

Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus Monkey, Pigtail Monkey, Rhesus Macaque, Sooty Mangabey, Squirrel Monkey

Format: cFluor^® BYG710

Isotype: IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG710 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.