PRODUCT DETAILS

Host: Mouse

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: pY20

Reactivity: Chem

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Numerous intracellular signaling cascades are propagated via phosphorylation of specific tyrosine on signaling proteins. The detection of p-Tyr residues is valuable for the characterization and purification of phosphorylated proteins and the biochemical pathways that they are involved in.

Applications Reported: This pY20 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and western blotting.

Applications Tested: This pY20 antibody has been tested by immunoblotting of mouse spleen cells left untreated or treated with the phosphatase inhibitor sodium orthovanadate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.