The Muse^® Human CD8 T Cell kit is used in laboratory research studies to determine the CD8+ T cell count, total lymphocyte count, and percent of CD8+ T cell lymphocytes in human whole blood and peripheral mononuclear cell (PBMC) samples. Identification and enumeration of cytotoxic CD8+ T cell populations are useful in understanding immune response and status, and in the research of disease mechanisms, such as autoimmune diseases and immunodeficiency.

The Muse Human CD8 T Cell kit allows for the detection and identification of lymphocytes and CD8+ T lymphocytes in both whole blood and PBMCs using a simplified, no-wash assay. The CD8 antibody allows the identification of CD8, a 68 kDa transmembrane glycoprotein expressed by class I major histocompatibility complex-restricted, mature suppressor/cytotoxic T cells, which are the majority of cortical thymocytes and approximately 30 percent of medullary thymocytes. Additionally, a portion of γδ T cells and NK cells express CD8. Inclusion of several proprietary antibodies in the anti-lymphocyte cocktail allows for the unique identification of the CD8+ cytotoxic T cells. Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Guava^® Muse^® cell analyzer provides:

• CD8 T cell concentration (cells/μL)
• CD8 T cell percent of lymphocytes
• Total lymphocyte concentration (cells/μL)

The Muse Human CD8 T Cell Kit is optimized for use with the Muse system, which makes sophisticated, fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse cell analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

PRODUCT DETAILS

Application:        Flow Cytometry

Storage:              See Documentation

Data Sheets:       QRC SDS

EXAMPLE DATA

Recommended Staining Protocol