PRODUCT DETAILS

Host: Mouse

Isotype: IgG2b, kappa

Clonality: Monoclonal

Clone: H127C

Reactivity: Hu

Application: Flow Cytometry

Tested Dilution: Assay-Dependent

Concentration: 0.5 mg/mL

Storage: 4°C

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The monoclonal antibody H127C recognizes human IL-38. IL-38 is a member of the Interleukin-1 family (IL-1F) and shares homology with IL-1R antagonist (IL-1Ra) and IL-36R antagonist (IL-36Ra). IL-38 is expressed mostly in the skin and proliferating B cells of the tonsil and is also present at low levels in the heart and placenta. IL-38 functions as an anti-inflammatory cytokine that inhibits IL-36 by binding to IL-36R and also inhibits the production of IL-17 and IL-22. Gene polymorphisms of IL-38 are associated with psoriatic arthritis, ankylosing spondylitis, and heart disease.

Applications Reported: This H127C antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, ELISA, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This H127C antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.