PRODUCT DETAILS

Host: Mouse

Isotype: IgM, kappa

Clonality: Monoclonal

Clone: HIS49

Format: FITC

Reactivity: Rt

Application: Flow Cytometry

Tested Dilution: 1 µg/test

Concentration: 0.5 mg/mL

Storage: 4°C, store in dark, DO NOT FREEZE!

Formulation: PBS with 0.09% sodium azide; pH 7.2

Purification: Affinity chromatography

Data Sheet: TDS

Specific Information

Description: The monoclonal antibody HIS49 reacts with an unknown antigen expressed by rat erythroid cells and their progenitors. HIS49 does not react with lymphocytes, monocytes or granulocytes.

Applications Reported: This HIS49 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIS49 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

 

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.