cFluor® R668 Anti-Human TCR γδ (B1)
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Description
The B1 monoclonal antibody binds to human γδ T cell receptor (TCR γδ). γδ TCR is a T cell receptor consisting of two glycoprotein chains, a 45-60 kDa gamma chain and a 40-60 kDa delta chain. Unlike conventional αβ T cell receptor, which recognizes peptide bound to MHC, γδ TCR recognizes antigen in non-MHC restricted way1,2. TCR γδ associates with CD3 to play a role in signal transduction and is found on thymocytes and subset of T cells. Some studies suggest its role in antigen presentation and autoimmune disease3.
The antibody was conjugated to a fluorophore and purified by affinity chromatography.
PRODUCT DETAILS
Clone: B1
Specificity: TCR γδ
Reactivity: Human, African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus
Format: cFluor® R6681
Isotype: Mouse IgG1, κ
Tested Dilution: 5 µL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
REFERENCES
1. Liu Y, et al. 2020. Cells. 13;9(5):1206
2. Kabelitz D, et al. 2000. Int Arch Allergy Immunol. 122(1):1-7
3. Bonneville M, et al. 2010, Nat Rev Immunol. 10(7):467-478
For research use only. Not intended for use in diagnostic procedures.
*cFluor® R668 is equivalent to CF®647 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.