The mouse monoclonal antibody clone 124-1D1 binds to human CD7, also known as gp40 or Leu-9. CD7 is a 40 kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is highly expressed on thymocytes and mature T cells and is also present on natural killer (NK) cells, hematopoietic progenitor cells, and a minor subset of monocytes. CD7 functions primarily as a costimulatory receptor. Engagement of CD7—including interaction with its characterized ligand SECTM1/K12—can enhance T-cell activation and proliferation and support NK-cell interferon-gamma secretion. CD7-mediated signaling involves PI3K-associated intracellular pathways. Clone 124-1D1 specifically recognizes human CD7 and is suitable for the identification and analysis of CD7-expressing lymphoid populations in flow cytometry and related immunoassays.

PRODUCT DETAILS

Clone: 124-1D1

Specificity: CD7

Reactivity: Human

Format: cFluor^® BYG610

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. van Dongen JJM, Lhermitte L, Böttcher S, et al. 2012. Cytometry A. 81A: 614–636.
2. Bene MC, Nebe T, Bettelheim P, et al. 1995. Leukemia. 9(10):1783–1786.
3. Orfao A, Schmitz G, Brando B, et al. 1999. Clin Chem Lab Med. 37(11–12):983–990.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

cFluor^® BYG610 is equivalent to RPE-CF^®596R manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.