violetFluor™ 500 Anti-Mouse CD8a (53-6.7)
The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
Kersh AE, Edwards LJ and Evavold BD. 2014. J. Immunol. September 29, 2014, doi:10.4049/jimmunol.1401456 [Epub ahead of print] (Flow Cytometry)
Finkin S, Yuan D, Stein I, Taniguchi K, Weber A, Unger K, Browning JL, Goossens N, Nakagawa S, Gunasekara G, Schwartz ME et al. 2015. Nat Immunol. doi: 10.1038/ni.3290. (Flow Cytometry)
|Name||violetFluor™ 500 Anti-Mouse CD8a (53-6.7)|
|Alternative Names||CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2|
|Isotype||Rat IgG2a, κ|