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C57Bl/6 splenocytes were stained with APC Anti-Mouse CD45R (B220) (20-0452) and 0.5 ug violetFluor™ 500 Anti-Mouse CD3 (85-0032) (right panel) or 0.5 ug violetFluor™ 500 Rat IgG2b isotype control (left panel).

violetFluor™ 500 Anti-Mouse CD3 (17A2)

SKU 85-0032-U025
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The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. Journal of Biological Chemistry. 287: 13324-13335).

Recent Publications:

Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.

» View on PubMed

Perez J, Dansou B, Herve R, Levi C, Tamouza H, Vandermeersch S, Demey-Thomas E, Haymann JP, Zafrani L, Klatzmann D, Boissier MC, Letavernier E and Baud L. 2015. J Immunol. doi: 10.4049/jimmunol.1500749. (Flow Cytometry)

Name violetFluor™ 500 Anti-Mouse CD3 (17A2)
Cat. No. 85-0032
Alternative Names CD3 epsilon
Gene ID 12502
Clone 17A2
Isotype Rat IgG2b, κ
Reactivity Mouse
Format violetFluor™ 500
Application Flow Cytometry
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