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C57Bl/6 splenocytes were stimulated overnight with ConA and then stained with 0.25 ug violetFluor™ 450 Anti-Mouse CD69 (75-0691) (solid line) or 0.25 ug violetFluor™ 450 Armenian hamster isotype control (dashed line).

violetFluor™ 450 Anti-Mouse CD69 (H1.2F3)

SKU 75-0691-U025
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The H1.2F3 antibody reacts with mouse CD69, a type II transmembrane glycoprotein also known as the Very Early Activation Antigen, EA-1, Leu23, Activation Inducer Molecule (AIM) and CLEC2C. CD69 is expressed as a 60 kDa disulfide-linked homodimer on activated T and B cells, NK cells, neutrophils and monocytes. Induction occurs rapidly upon activation. It is also constitutively expressed on platelets and a subset of thymocytes. CD69 acts as a co-stimulatory molecule involved in activation and proliferation of T cells, and may be a marker for thymocytes undergoing TCR-mediated positive selection.

Co-stimulation with the H1.2F3 clone has been reported to enhance T cell and macrophage activation. Please choose the appropriate format for each application.

Name violetFluor™ 450 Anti-Mouse CD69 (H1.2F3)
Cat. No. 75-0691
Alternative Names Very Early Activation Antigen (VEA), AIM, EA-1, Leu23, CLEC2C, MLR3, gp34/28
Gene ID 12515
Clone H1.2F3
Isotype Armenian Hamster IgG
Reactivity Mouse
Format violetFluor™ 450
Application Flow Cytometry
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