violetFluor™ 450 Anti-Mouse CD3 (17A2)
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The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.
The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. Journal of Biological Chemistry. 287: 13324-13335).
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Ishida Y, Kuninaka Y, Nosaka M, et al. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis. Nat Commun. 2020 Nov 25;11(1):5994. doi: 10.1038/s41467-020-19763-0. PMID: 33239616.
Andersen TK, Huszthy PC, Gopalakrishnan RP, et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ Vaccines. 2019;4:9. Published 2019 Feb 11. doi:10.1038/s41541-019-0101-0.
Perez J, Dansou B, Herve R, Levi C, Tamouza H, Vandermeersch S, Demey-Thomas E, Haymann JP, Zafrani L, Klatzmann D, Boissier MC, Letavernier E and Baud L. 2015. J Immunol. doi: 10.4049/jimmunol.1500749. (Flow Cytometry)
|Name||violetFluor™ 450 Anti-Mouse CD3 (17A2)|
|Alternative Names||CD3 epsilon|
|Isotype||Rat IgG2b, κ|