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C57Bl/6 splenocytes were stained with 0.5 ug violetFluor™ 450 Anti-Hu/Mo CD45R (B220) (75-0452) (solid line) or 0.5 ug violetFluor™ 450 Rat IgG2a isotype control (dashed line).

violetFluor™ 450 Anti-Human/Mouse CD45R (B220) (RA3-6B2)

SKU 75-0452-U025
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The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.


Recent Citations:

Kotake K, Kumazawa T, Adachi T. Long-term administration of Tetragenococcus halophilus No. 1 over generations affects the immune system of mice. PLoS One. 2022 Apr 26;17(4):e0267473. doi: 10.1371/journal.pone.0267473. PMID: 35472068; PMCID: PMC9041805.

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Hong H, Lee J, Park GY, Kim S, Park J, Park JS, Song Y, Lee S, Kim TJ, Lee YJ, Roh TY, Kwok SK, Kim SW, Tan Q, Lee Y. Postnatal regulation of B-1a cell development and survival by the CIC-PER2-BHLHE41 axis. Cell Rep. 2022 Feb 15;38(7):110386. doi: 10.1016/j.celrep.2022.110386. PMID: 35172136.

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Simões IT, Aranda F, Casadó-Llombart S, et al. Multifaceted effects of soluble human CD6 in experimental cancer models. J Immunother Cancer. 2020 Mar;8(1):e000172. doi: 10.1136/jitc-2019-000172. PMID: 32217757; PMCID: PMC7174071.

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Name violetFluor™ 450 Anti-Human/Mouse CD45R (B220) (RA3-6B2)
Cat. No. 75-0452
Alternative Names Ly-5, Lyt-4, T200
Gene ID 19264
Clone RA3-6B2
Isotype Rat IgG2a, κ
Reactivity Human, Mouse
Cross Reactivity Feline
Format violetFluor™ 450
Application Flow Cytometry
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