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Human peripheral blood lymphocytes were stained with 5 uL (1 ug) violetFluor™ 450 Anti-Human CD8a (75-0089) (solid line) or 1 ug violetFluor™ 450 Mouse IgG1 isotype control (dashed line).

violetFluor™ 450 Anti-Human CD8a (Hit8a)

SKU 75-0089-T025
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The Hit8a antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

The Hit8a antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with an alternative Anti-Human CD8a clone RPA-T8, the Hit8a antibody will not block binding of RPA-T8 to CD8a.

Name violetFluor™ 450 Anti-Human CD8a (Hit8a)
Cat. No. 75-0089
Alternative Names CD8 alpha, leu-2a
Gene ID 925
Clone Hit8a
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Chimpanzee, Rhesus
Format violetFluor™ 450
Application Flow Cytometry
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