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Human peripheral blood lymphocytes were stained with 5 uL (0.5 ug) violetFluor™ 450 Anti-Human CD45RA (75-0458) (solid line) or 0.5 ug violetFluor™ 450 Mouse IgG2b isotype control (dashed line).

violetFluor™ 450 Anti-Human CD45RA (HI100)

SKU 75-0458-T025
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The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB and CD45RO.

The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.

Recent Publications:

Zhang AH, Yoon J, Kim YC, Scott DW. Targeting Antigen-Specific B Cells Using Antigen-Expressing Transduced Regulatory T Cells. J Immunol. 2018;201(5):1434-1441. doi:10.4049/jimmunol.1701800.

Schumann K, Lin S, Boyer E, Simeonov DR, Subramaniam M, Gate RE, Haliburton GE, Ye CJ, Blueston JA, Doudna JA and Marson A. 2015. Proc Natl Acad Sci USA. DOI: 10.1073/pnas.1512503112. (Flow Cytometry)

Name violetFluor™ 450 Anti-Human CD45RA (HI100)
Cat. No. 75-0458
Gene ID 5788
Clone HI100
Isotype Mouse IgG2b, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format violetFluor™ 450
Application Flow Cytometry
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