Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)
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Description
The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.
Recent Publications:
Picarda E, Galbo PM Jr, Zong H, Rajan MR, Wallenius V, Zheng D, Börgeson E, Singh R, Pessin J, Zang X. The immune checkpoint B7-H3 (CD276) regulates adipocyte progenitor metabolism and obesity development. Sci Adv. 2022 Apr 29;8(17):eabm7012. doi: 10.1126/sciadv.abm7012. Epub 2022 Apr 27. PMID: 35476450; PMCID: PMC9045715.
Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R. RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. Nat Commun. 2022 Apr 20;13(1):2155. doi: 10.1038/s41467-022-29869-2. PMID: 35444206; PMCID: PMC9021285.
» View on NatureCommunications
Carson CS, Becker KW, Garland KM, Pagendarm HM, Stone PT, Arora K, Wang-Bishop L, Baljon JJ, Cruz LD, Joyce S, Wilson JT. A nanovaccine for enhancing cellular immunity via cytosolic Co-delivery of antigen and PolyIC RNA. J Control Release. 2022 Mar 14:S0168-3659(22)00146-8. doi: 10.1016/j.jconrel.2022.03.020. Epub ahead of print. PMID: 35301055.
Yuan M, Zhai Y, Men Y, Zhao M, Sun X, Ma Z, Bao Y, Yang X, Sun S, Liu Y, Zhang W, Hui Z. Anlotinib Enhances the Antitumor Activity of High-Dose Irradiation Combined with Anti-PD-L1 by Potentiating the Tumor Immune Microenvironment in Murine Lung Cancer. Oxid Med Cell Longev. 2022 Feb 1;2022:5479491. doi: 10.1155/2022/5479491. PMID: 35154567; PMCID: PMC8825674.
Kang Y, Lim J, Saravanakumar G, Kim J, Park M, Im S, Kim WJ. Immunostimulation of tumor microenvironment by targeting tumor-associated macrophages with hypoxia-responsive nanocomplex for enhanced anti-tumor therapy. J Control Release. 2022 Jan 19;343:78-88. doi: 10.1016/j.jconrel.2022.01.021. Epub ahead of print. PMID: 35065158.
Brandi P, Conejero L, Cueto FJ, Martínez-Cano S, Dunphy G, Gómez MJ, Relaño C, Saz-Leal P, Enamorado M, Quintas A, Dopazo A, Amores-Iniesta J, Del Fresno C, Nistal-Villán E, Ardavín C, Nieto A, Casanovas M, Subiza JL, Sancho D. Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections. Cell Rep. 2022 Jan 4;38(1):110184. doi: 10.1016/j.celrep.2021.110184. PMID: 34986349.
Hernández-García E, Cueto FJ, Cook ECL, Redondo-Urzainqui A, Charro-Zanca S, Robles-Vera I, Conde-Garrosa R, Nikolić I, Sabio G, Sancho D, Iborra S. Conventional type 1 dendritic cells protect against age-related adipose tissue dysfunction and obesity. Cell Mol Immunol. 2022 Jan 4. doi: 10.1038/s41423-021-00812-7. Epub ahead of print. PMID: 34983945.
Ishikura N, Sugimoto M, Yorozu K, Kurasawa M, Kondoh O. Anti‑VEGF antibody triggers the effect of anti‑PD‑L1 antibody in PD‑L1low and immune desert‑like mouse tumors. Oncol Rep. 2022 Feb;47(2):36. doi: 10.3892/or.2021.8247. Epub 2021 Dec 27. PMID: 34958105.
Raihan MO, McGregor BA, Velaris NA, Brishti A, Hur J, Porter JE. Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse. Methods Protoc. 2021 Dec 2;4(4):86. doi: 10.3390/mps4040086. PMID: 34940397; PMCID: PMC8704779.
Oda Y, Takahashi C, Harada S, Nakamura S, Sun D, Kiso K, Urata Y, Miyachi H, Fujiyoshi Y, Honigmann A, Uchida S, Ishihama Y, Toyoshima F. Discovery of anti-inflammatory physiological peptides that promote tissue repair by reinforcing epithelial barrier formation. Sci Adv. 2021 Nov 19;7(47):eabj6895. doi: 10.1126/sciadv.abj6895. Epub 2021 Nov 17. PMID: 34788088.
Bickett TE, Knitz MW, Piper M, Oweida AJ, Gadwa J, Darragh LB, Nguyen D, Bhatia S, Bhuvane S, Phan AV, Van Court B, Corbo S, Pham T, Dent AL, Lenz L, Karam SD. Dichotomous Effects of Cellular Expression of STAT3 on Tumor Growth of HNSCC. Mol Ther. 2021 Nov 15:S1525-0016(21)00582-7. doi: 10.1016/j.ymthe.2021.11.011. Epub ahead of print. PMID: 34793974.
Winkels H, Ghosheh Y, Kobiyama K, Kiosses WB, Orecchioni M, Ehinger E, Suryawanshi V, Herrera-De La Mata S, Marchovecchio P, Riffelmacher T, Thiault N, Kronenberg M, Wolf D, Seumois G, Vijayanand P, Ley K. Thymus-Derived CD4+CD8+ Cells Reside in Mediastinal Adipose Tissue and the Aortic Arch. J Immunol. 2021 Nov 5:ji2100208. doi: 10.4049/jimmunol.2100208. Epub ahead of print. PMID: 34740961.
» View on The Journal of Immunology
Stephens WZ, Kubinak JL, Ghazaryan A, Bauer KM, Bell R, Buhrke K, Chiaro TR, Weis AM, Tang WW, Monts JK, Soto R, Ekiz HA, O'Connell RM, Round JL. Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition. Cell Rep. 2021 Nov 2;37(5):109916. doi: 10.1016/j.celrep.2021.109916. PMID: 34731608.
Monteith AJ, Miller JM, Maxwell CN, Chazin WJ, Skaar EP. Neutrophil extracellular traps enhance macrophage killing of bacterial pathogens. Sci Adv. 2021 Sep 10;7(37):eabj2101. doi: 10.1126/sciadv.abj2101. Epub 2021 Sep 10. PMID: 34516771.
Takai J, Shimada T, Nakamura T, Engel JD, Moriguchi T. Gata2 heterozygous mutant mice exhibit reduced inflammatory responses and impaired bacterial clearance. iScience. 2021 Jul 29;24(8):102836. doi: 10.1016/j.isci.2021.102836. PMID: 34471858; PMCID: PMC8390858.
Pingili AK, Chaib M, Sipe LM, et al. Immune checkpoint blockade reprograms systemic immune landscape and tumor microenvironment in obesity-associated breast cancer. Cell Rep. 2021 Jun 22;35(12):109285. doi: 10.1016/j.celrep.2021.109285. PMID: 34161764.
Xu C, Sun S, Johnson T, Qi R, Zhang S, Zhang J, Yang K. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity. Cell Rep. 2021 Jun 15;35(11):109235. doi: 10.1016/j.celrep.2021.109235. PMID: 34133924.
Lei Y, Guerra Martinez C, Torres-Odio S, et al. Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice. Sci Adv. 2021 May 26;7(22):eabe7548. doi: 10.1126/sciadv.abe7548. PMID: 34039599.
Yan J, Zhao Q, Wang J, Tian X, Wang J, Xia X, Ott M, Rao G, Heimberger AB, Li S. FGL2-wired macrophages secrete CXCL7 to regulate the stem-like functionality of glioma cells. Cancer Lett. 2021 May 28;506:83-94. doi: 10.1016/j.canlet.2021.02.021. Epub 2021 Mar 4. PMID: 33676940; PMCID: PMC8009861.
Knitz MW, Bickett TE, Darragh LB, et al. Targeting resistance to radiation-immunotherapy in cold HNSCCs by modulating the Treg-dendritic cell axis. J Immunother Cancer. 2021 Apr;9(4):e001955. doi: 10.1136/jitc-2020-001955. PMID: 33883256.
Lee W, Kingstad-Bakke B, Paulson B, et al. Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells. PLoS Pathog. 2021 Jan 14;17(1):e1009168. doi: 10.1371/journal.ppat.1009168. PMID: 33444400.
Prokopi A, Tripp CH, Tummers B, et al. Skin dendritic cells in melanoma are key for successful checkpoint blockade therapy. J Immunother Cancer. 2021 Jan;9(1):e000832. doi: 10.1136/jitc-2020-000832. PMID: 33408092.
C Khouili S, Cook ECL, Hernández-García E, et al. SHP-1 Regulates Antigen Cross-Presentation and Is Exploited by Leishmania to Evade Immunity. Cell Rep. 2020 Dec 1;33(9):108468. doi: 10.1016/j.celrep.2020.108468. PMID: 33264612.
Castillo-González R, Cibrian D, Fernández-Gallego N, et al. Galectin-1 expression in CD8+ T lymphocytes controls inflammation in contact hypersensitivity. J Invest Dermatol. 2020 Nov 9:S0022-202X(20)32281-8. doi: 10.1016/j.jid.2020.10.020. PMID: 33181141.
Baniel CC, Sumiec EG, Hank JA, et al. Intratumoral injection reduces toxicity and antibody-mediated neutralization of immunocytokine in a mouse melanoma model. J Immunother Cancer. 2020 Oct;8(2):e001262. doi: 10.1136/jitc-2020-001262. PMID: 33115944.
Marinaik CB, Kingstad-Bakke B, Lee W, Hatta M, Sonsalla M, Larsen A, Neldner B, Gasper DJ, Kedl RM, Kawaoka Y, Suresh M. Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants. Cell Rep Med. 2020 Sep 22;1(6):100095. doi: 10.1016/j.xcrm.2020.100095. PMID: 32984856; PMCID: PMC7508055.
Innamarato P, Asby S, Morse J, et al. Intratumoral Activation of 41BB Costimulatory Signals Enhances CD8 T Cell Expansion and Modulates Tumor-Infiltrating Myeloid Cells. J Immunol. 2020 Oct 5:ji2000759. doi: 10.4049/jimmunol.2000759. PMID: 33020146.
Shen M, Song Y, Ichinose T, et al. In vivo immune activation of splenocytes following exposure to tar from Asian sand dust. J Toxicol Environ Health A. 2020;1-10. doi:10.1080/15287394.2020.1806160.
Yamamuro T, Kawabata T, Fukuhara A, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11(1):4150. Published 2020 Aug 18. doi:10.1038/s41467-020-17985-w.
» View on Nature Communications
Wu B, Chiang HC, Sun X, et al. Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation. J Immunother Cancer. 2020;8(2):e000964. doi:10.1136/jitc-2020-000964.
Kajihara N, Kitagawa F, Kobayashi T, Wada H, Otsuka R, Seino KI. Interleukin-34 contributes to poor prognosis in triple-negative breast cancer [published online ahead of print, 2020 Jun 23]. Breast Cancer. 2020;10.1007/s12282-020-01123-x. doi:10.1007/s12282-020-01123-x.
Wormsbaecher C, Hindman AR, Avendano A, et al. In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness. Breast Cancer Res. 2020;22(1):41. Published 2020 May 5. doi:10.1186/s13058-020-01275-w.
McDaniel MM, Kottyan LC, Singh H, Pasare C. Suppression of Inflammasome Activation by IRF8 and IRF4 in cDCs Is Critical for T Cell Priming. Cell Rep. 2020 May 5;31(5):107604. doi: 10.1016/j.celrep.2020.107604
Markman, J.L., Porritt, R.A., Wakita, D. et al. Loss of testosterone impairs anti-tumor neutrophil function. Nat Commun 11, 1613 (2020). https://doi.org/10.1038/s41467-020-15397-4.
» View on Nature Communications
Adam L. Burrack, Ellen J. Spartz, Jackson F. Raynor, Iris Wang, Margaret Olson, Ingunn M. Stromnes. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019 Aug 20;28(8):2140-2155.e6. doi: 10.1016/j.celrep.2019.07.059
» View on PubMed
Kim IS, Gao Y, Welte T, et al. Immuno-subtyping of breast cancer reveals distinct myeloid cell profiles and immunotherapy resistance mechanisms. Nat Cell Biol. 2019;21(9):1113-1126. doi:10.1038/s41556-019-0373-7.
Sarika Saraswati, Stephanie M.W. Marrow, Lester A. Watch1 & Pampee P. Young. Identification of a pro-angiogenic functional role for FSP1-positive fibroblast subtype in wound healing. Nat Commun. 2019 Jul 9;10(1):3027. doi: 10.1038/s41467-019-10965-9
» View on PubMed
Yang BH, Wang K, Wan S, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27(12):3629-3645.e6. doi:10.1016/j.celrep.2019.05.061.
Dutta R, Lunzer MM, Auger JL, Akgün E, Portoghese PS, Binstadt BA. A bivalent compound targeting CCR5 and the mu opioid receptor treats inflammatory arthritis pain in mice without inducing pharmacologic tolerance. Arthritis Res Ther. 2018;20(1):154. Published 2018 Jul 27. doi:10.1186/s13075-018-1661-5.
Guo M, Price MJ, Patterson DG, et al. EZH2 Represses the B Cell Transcriptional Program and Regulates Antibody-Secreting Cell Metabolism and Antibody Production. J Immunol. 2018;200(3):1039-1052. doi:10.4049/jimmunol.1701470.
Adami AJ, Bracken SJ, Guernsey LA, et al. Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma. Pediatr Res. 2018;84(3):426-434. doi:10.1038/s41390-018-0031-y.
Grinberg-Bleyer Y, Oh H, Desrichard A, Bhatt DM, Caron R, Chan TA, Schmid RM, Klein U, Hayden MS and Ghosh S. 2017. NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer. Cell. doi: 10.1016/j.cell.2017.08.004.
Benck CJ, Martinov T, Fife BT and Chatterjea D. 2016. J. Vis. Exp. (107), e53638, doi:10.3791/53638. (Blocking - Flow Cytometry)
| Name | Purified Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2) |
|---|---|
| Cat. No. | 70-0161 |
| Alternative Names | FCGR3, IGFR3; FCGR2, IGFR2; FC Receptor |
| Gene ID | 14130/14131 |
| Clone | 2.4G2 |
| Isotype | Rat IgG2b |
| Reactivity | Mouse |
| Format | Purified |
| Application | Flow Cytometry, IF, IP |