The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

PRODUCT DETAILS

Clone: HHF35

Specificity: Actin (Muscle)

Reactivity: Human|Mouse, Non-Human Primate, Canine, Chicken, Feline, Rabbit, Rat

Format: Purified

Isotype: Mouse IgG1

Application: WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

REFERENCES

1. Matsuyama A, et al. 2007. Hum Pathol 38:645-51.
2. Matsuo S, et al. 2007. J Surg Oncol 96:633-6.
3. Tsukada T, et al. 1987. Am J Pathol 126:51-60.
4. Tsukada T, et al. 1987. Am J Pathol 127:389-402.
5. Schmidt RA, et al. 1988. Am J Pathol 131:19-28.
6. Miettinen M. 1988. Am J Pathol 130:205-15.
7. de Sousa SO, et al. 2000. J Clin Pathol 53:153-6.
8. Handharyani E, et al. 2001. J Vet Med Sci 63:547-52.
9. Ijzer J, et al. 2006. Comp Hepatol 5:7.

For research use only. Not intended for use in diagnostic procedures.