The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

PRODUCT DETAILS

Clone: 1H4

Specificity: CD54 (ICAM-1)

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2b

Application: FC, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required.
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.

REFERENCES

1. Boyd AW, et al. 1988. Proc Natl Acad Sci U S A 85:3095-9.
2. Boyd AW, et al. 1989. Blood 73:1896-903.
3. Springer TA. 1990. Nature 346:425-34.
4. Ockenhouse CF, et al. 1992. Cell 68:63-9.
5. Williams DT, et al. 2004. J Gen Virol 85:731-738.

For research use only. Not intended for use in diagnostic procedures.