The mouse monoclonal antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.

PRODUCT DETAILS

Clone: B-A8

Specificity: CD14

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: ELISA, IHC-P, FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18). Flow cytometry: recommended dilution: 1 μg/ml; Immunohistochemistry (paraffin sections): recommended dilution: 2-10 μg/ml, positive tissue: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.

REFERENCES

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4. Fernández-Real JM, et al. 2003. J Clin Endocrinol Metab 88:1780-4.
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6. Matasić R, et al. 1999. J Leukoc Biol 66:909-14.
7. Kuusniemi AM, et al. 2005. Kidney Int 68:121-32.
8. Goyert SM, et al. 1988. Science 239:497-500.
9. Yates J, et al. 2007. Int Immunol 19:785-99.

For research use only. Not intended for use in diagnostic procedures.