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Propidium Iodide
SKU 13-6990-T200
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Description
Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.
| Name | Propidium Iodide |
|---|---|
| Cat. No. | 13-6990 |
| Protocol | Propidium Iodide or 7-AAD Viability Staining Protocol |
| Excitation Laser | Blue (488 nm), Green (532 nm), Yellow-Green (561 nm) |
| Emission (nm) | 535 - 617 |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2 |
| Application | Flow Cytometry |