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Propidium Iodide

Propidium Iodide

SKU 13-6990-T200
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Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.  
Name Propidium Iodide
Cat. No. 13-6990
Protocol Propidium Iodide or 7-AAD Viability Staining Protocol
Excitation Laser Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
Emission (nm) 535 - 617
Formulation 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Application Flow Cytometry
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