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C57Bl/6 (left panel) or SJL (right panel) splenocytes were stained with 0.5 ug PerCP-Cy5.5 Anti-Mouse CD45.1 (65-0453) (solid line) or 0.5 ug PerCP-Cy5.5 Mouse IgG2a isotype control (dashed line).

PerCP-Cyanine5.5 Anti-Mouse CD45.1 (A20)

SKU 65-0453-U025
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The A20 antibody reacts with mouse CD45.1, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.

The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Recent Publications:

Mishra S, Liao W, Liu Y, Yang M, Ma C, Wu H, Zhao M, Zhang X, Qiu Y, Lu Q, Zhang N. TGF-β and Eomes control the homeostasis of CD8+ regulatory T cells. J Exp Med. 2021 Jan 4;218(1):e20200030. doi: 10.1084/jem.20200030. PMID: 32991667; PMCID: PMC7527976.

» View on PubMed

Lin CC, Bradstreet TR, Schwarzkopf EA, Jarjour NN, Chou C, Archambault AS, Sim J, Zinselmeyer BH, Carrero JA, Wu GF, Taneja R, Artyomov MN, Russell JH and Edelson BT. 2016. J Exp Med. 213(2): 251-271. (Flow Cytometry)

Name PerCP-Cyanine5.5 Anti-Mouse CD45.1 (A20)
Cat. No. 65-0453
Alternative Names SJL, Ly5.1
Gene ID 19264
Clone A20
Isotype Mouse IgG2a, κ
Reactivity Mouse
Format PerCP-Cyanine5.5
Application Flow Cytometry
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