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C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD3e (65-0031) (solid line) or 0.25 ug PerCP-Cy5.5 Armenian hamster IgG isotype control (dashed line).

PerCP-Cyanine5.5 Anti-Mouse CD3e (145-2C11)

SKU 65-0031-U025
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The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

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Wu B, Zhang X, Chiang HC, Pan H, Yuan B, Mitra P, Qi L, Simonyan H, Young CN, Yvon E, Hu Y, Zhang N, Li R. RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. Nat Commun. 2022 Apr 20;13(1):2155. doi: 10.1038/s41467-022-29869-2. PMID: 35444206; PMCID: PMC9021285.

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Forte D, García-Fernández M, Sánchez-Aguilera A, Stavropoulou V, Fielding C, Martín-Pérez D, López JA, Costa ASH, Tronci L, Nikitopoulou E, Barber M, Gallipoli P, Marando L, Fernández de Castillejo CL, Tzankov A, Dietmann S, Cavo M, Catani L, Curti A, Vázquez J, Frezza C, Huntly BJ, Schwaller J, Méndez-Ferrer S. Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy. Cell Metab. 2020 Sep 16:S1550-4131(20)30479-4. doi: 10.1016/j.cmet.2020.09.001. PMID: 32966766.   

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Name PerCP-Cyanine5.5 Anti-Mouse CD3e (145-2C11)
Cat. No. 65-0031
Alternative Names CD3 epsilon
Gene ID 12501
Clone 145-2C11
Isotype Armenian Hamster IgG
Reactivity Mouse
Format PerCP-Cyanine5.5
Application Flow Cytometry
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