The antibody MEM-241 recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T-cells) and also on monocytes, tissue macrophages and granulocytes.

PRODUCT DETAILS

Clone: MEM-241

Specificity: CD4

Reactivity: Human

Format: PerCP-Cyanine5.5

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Millán J, et al. 1999. Tissue Antigens 53:33-40.
2. Brdicková N, et al. 2003. J Exp Med 198:1453-62.
3. Karlsson KR, et al. 2008. Exp Hematol 36:1167-75.
4. Manasa J, et al. 2007. Clin Vaccine Immunol 14:293-8.
5. Anderson AE, et al. 2008. J Leukoc Biol 84:124-33.
6. Hovden AO, et al. 2011. BMC Immunol 12:2.
7. Zola H, et al. 2007. J Immunol Methods 319:1-5.
8. Kanderova V, et al. 2016. Mol Cell Proteomics 15:1246-61.
9. Ohradanova-Repic A, et al. 2018. Front Immunol 9:852.
10. Černý V, et al. 2021. Biomedicines 9.

For research use only. Not intended for use in diagnostic procedures.

PerCP-Cyanine5.5 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.