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Human peripheral blood lymphocytes were stained with 5 uL (0.25 ug) PerCP-Cy5.5 Anti-Human CD3 (65-0037) (solid line) or 0.25 ug PerCP-Cy5.5 Mouse IgG2a isotype control (dashed line).

PerCP-Cyanine5.5 Anti-Human CD3 (OKT3)

SKU 65-0037-T025
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$82.00

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Description

TDS | SDS

The OKT3 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The OKT3 antibody is a widely used phenotypic marker for human T cells. In addition, as the CD3e subunit of the TCR complex contains intracellular signaling domains, binding of OKT3 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a CD3e-mitogenic antibody complex provides further insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the widely used human CD3 antibodies OKT3 and UCHT1 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335). OKT3 has also been shown to be cross-reactive with Chimpanzee CD3 and has been used for in vitro activation of T cells in this species. Please choose the appropriate format for each application.

Recent Publications:
Bachanova V, Frankel AE, Cao Q, Lewis D, Grzywacz B, Verneris MR, Ustun C, Lazaryan A, McClune B, Warlick ED, Kantarjian H, Weisdorf DJ, Miller JS, Vallera DA. 2015. Clin Cancer Res. 21(6): 1267-1272. (Flow Cytometry)

Name PerCP-Cyanine5.5 Anti-Human CD3 (OKT3)
Cat. No. 65-0037
Alternative Names Leu-4, T3
Gene ID 916
Clone OKT3
Isotype Mouse IgG2a, κ
Reactivity Human
Cross Reactivity Chimpanzee
Format PerCP-Cyanine5.5
Application Flow Cytometry
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