The mouse monoclonal antibody clone WM15 binds to human CD13, a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin, including granulocytes, monocytes, mast cells, and GM-progenitor cells. The majority of acute myeloid leukemia (AML) cases and chronic myeloid leukemia (CML) cases—particularly during the myeloid blast crisis phase—express this marker, whereas it is present in only a smaller proportion of lymphoid leukemias. CD13 is also present on fibroblasts, endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is absent from normal lymphocytes, platelets, and erythrocytes. CD13 is reported to have many different functions, including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells. CD13 is also reported to serve as a receptor for some strains of human coronaviruses (HCV).

PRODUCT DETAILS

Clone: WM15

Specificity: CD13

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque

Format: PerCP-Cyanine5.5

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Favaloro EJ, et al. 1987. Dis Markers. 5(4):215-225.
2. Nakamura Y, et al. 1994. Blood. 83(5):1442-3.
3. Freeman SD, et al. 1995. Blood. 85(8):2005-2012.
4. Walter RB, et al. 2007. Traffic. 9(2):267-279.

For research use only. Not intended for use in diagnostic procedures.

PerCP-Cyanine5.5 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.