The mouse monoclonal antibody clone 6H6 binds to human CD123, a 70-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily, also known as the Interleukin-3 receptor alpha chain. CD123 alone has a low affinity to IL-3 and develops a high affinity when associated with βc (common chain). The CD123 molecule is expressed predominantly on hematopoietic progenitor cells, macrophages, dendritic cells, basophils, eosinophils, monocytes, megakaryocytes, and some B cells. It is also present in myeloid, lymphoid, and vascular endothelial cells.

PRODUCT DETAILS

Clone: 6H6

Specificity: CD123

Reactivity: Human, Rhesus Macaque

Format: PerCP-Cyanine5.5

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Sun Q, et al. 1996. Blood. 87:83-92.
2. Miyajima A. 1997. Leucocyte typing VI.854-855.

For research use only. Not intended for use in diagnostic procedures.

PerCP-Cyanine5.5 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.