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C57Bl/6 splenocytes were stained with APC Anti-Mouse CD3 (20-0031) and 0.5 ug PerCP Anti-Human/Mouse CD45R (B220) (67-0452) (right panel) or 0.5 ug PerCP Rat IgG2a isotype control (left panel).

PerCP Anti-Human/Mouse CD45R (B220) (RA3-6B2)

SKU 67-0452-U025
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$84.00

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Description

TDS | SDS

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Name PerCP Anti-Human/Mouse CD45R (B220) (RA3-6B2)
Cat. No. 67-0452
Alternative Names Ly-5, Lyt-4, T200
Gene ID 19264
Clone RA3-6B2
Isotype Rat IgG2a, κ
Reactivity Human, Mouse
Cross Reactivity Feline
Format PerCP
Application Flow Cytometry
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