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C57Bl/6 splenocytes were stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel) and then stained with FITC Anti-Mouse CD3e (35-0031), followed by intracellular staining with 0.125 ug PE-Cy7 Anti-Mouse IFN gamma (60-7311).

PE-Cyanine7 Anti-Mouse IFN gamma (XMG1.2)

SKU 60-7311-U025
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The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).

The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).


Recent Citations:

Trott DW, Islam MT, Buckley DJ, Donato AJ, Dutson T, Sorensen ES, Cai J, Gogulamudi VR, Phuong TTT, Lesniewski LA. T lymphocyte depletion ameliorates age-related metabolic impairments in mice. Geroscience. 2021 Apr 24. doi: 10.1007/s11357-021-00368-4. PMID: 33893902.

» View on PubMed

Name PE-Cyanine7 Anti-Mouse IFN gamma (XMG1.2)
Cat. No. 60-7311
Alternative Names IFN-g, IFNg, Interferon-g, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Gene ID 15978
Clone XMG1.2
Isotype Rat IgG1, kappa
Reactivity Mouse
Format PE-Cyanine7
Application Flow Cytometry
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