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C57Bl/6 splenocytes were stained with 0.25 ug PE-Cy7 Anti-Mouse CD4 (60-0041) (solid line) or 0.25 ug PE-Cy7 Rat IgG2b isotype control (dashed line).

PE-Cyanine7 Anti-Mouse CD4 (GK1.5)

SKU 60-0041-U025
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The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.


Recent Publications:

Prakhar P, Gonzalez V, Park JH. High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM+ cells. STAR Protoc. 2022 Feb 25;3(1):101207. doi: 10.1016/j.xpro.2022.101207. PMID: 35243383; PMCID: PMC8885746.

» View on PubMed

Chun N, Ang RL, Chan M, Fairchild RL, Baldwin Iii WM, Horwitz JK, Gelles JD, Chipuk JE, Kelliher MA, Pavlov VI, Li Y, Homann D, Heeger PS, Ting AT. T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death. JCI Insight. 2021 Nov 9:e148643. doi: 10.1172/jci.insight.148643. Epub ahead of print. PMID: 34752416.

» View on PubMed

Li C, Park JH. Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3+ Treg Cells by Multiparameter Flow Cytometry. STAR Protoc. 2020 Dec 1;1(3):100195. doi: 10.1016/j.xpro.2020.100195. PMID: 33377089; PMCID: PMC7757559.

» View on PubMed

Name PE-Cyanine7 Anti-Mouse CD4 (GK1.5)
Cat. No. 60-0041
Alternative Names L3T4, T4
Gene ID 12504
Clone GK1.5
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity Syrian Hamster
Format PE-Cyanine7
Application Flow Cytometry
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