Shopping Cart
C57Bl/6 splenocytes were stained with 1 ug PE-Cy7 Anti-Mouse CD3e (60-0031) (solid line) or 1 ug PE-Cy7 Armenian hamster IgG isotype control (dashed line).

PE-Cyanine7 Anti-Mouse CD3e (145-2C11)

SKU 60-0031-U025
Regular price

$53.00

task_alt Added to cart.
View Cart or Continue shopping.
Description

TDS | SDS

The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

 

Recent Citations:

Dikiy S, Li J, Bai L, Jiang M, Janke L, Zong X, Hao X, Hoyos B, Wang ZM, Xu B, Fan Y, Rudensky AY, Feng Y. A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance. Immunity. 2021 May 11;54(5):931-946.e11. doi: 10.1016/j.immuni.2021.03.020. Epub 2021 Apr 9. PMID: 33838102; PMCID: PMC8317508.

 » View on PubMed

Name PE-Cyanine7 Anti-Mouse CD3e (145-2C11)
Cat. No. 60-0031
Alternative Names CD3 epsilon
Gene ID 12501
Clone 145-2C11
Isotype Armenian Hamster IgG
Reactivity Mouse
Format PE-Cyanine7
Application Flow Cytometry
Shipping
Price calculated at checkout.