The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

PRODUCT DETAILS

Clone: MEM-181

Specificity: CD25

Reactivity: Human

Format: PE-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Sotlar K, et al. 2004. Am J Surg Pathol 28:1319-25.
2. Morris JC, et al. 2000. Ann Rheum Dis 59 Suppl 1:i109-14.
3. Scheibenbogen C, et al. 1992. Res Immunol 143:33-7.
4. Lai KN, et al. 1991. Pathology 23:224-8.
5. Drbal K, et al. 2007. Int Immunol 19:675-84.
6. Černý V, et al. 2018. PLoS One 13:e0207998.
7. Černý V, et al. 2021. Biomedicines 9.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.