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Human peripheral blood lymphocytes were stained with FITC Anti-Human CD4 (35-0048) and 5 uL (0.25 ug) PE-Cy7 Anti-Human CD25 (60-0259) (right panel) or 0.25 ug PE-Cy7 Mouse IgG1 isotype control (left panel).

PE-Cyanine7 Anti-Human CD25 (BC96)

SKU 60-0259-T025
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The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.

The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Recent Publications:

Zhang AH, Yoon J, Kim YC, Scott DW. Targeting Antigen-Specific B Cells Using Antigen-Expressing Transduced Regulatory T Cells. J Immunol. 2018;201(5):1434-1441. doi:10.4049/jimmunol.1701800.

Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T et al. 2015. Nat Commun. doi: 10.1038/ncomms9608. (Flow Cytometry)

Name PE-Cyanine7 Anti-Human CD25 (BC96)
Cat. No. 60-0259
Alternative Names Interleukin-2 Receptor alpha, IL-2Rα, IL2Ra, Tac
Gene ID 3559
Clone BC96
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Baboon, Chimpanzee, Cynomolgus, Rhesus
Format PE-Cyanine7
Application Flow Cytometry
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