The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.

PRODUCT DETAILS

Clone: 55-7H1

Specificity: CD144 (VE-Cadherin)

Reactivity: Human

Format: PE-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Dejana E, et al. 2013. J Cell Sci 126:2545-9.
2. Tisato V, et al. 2012. PLoS One 7:e39543.
3. De Ugarte DA, et al. 2003. Immunol Lett 89:267-70.
4. Limaye V, et al. 2005. Blood 105:3169-77.
5. Hamilton RD, et al. 2007. J Anat 211:707-16.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.