The mouse monoclonal antibody clone WM53 binds to human CD33, a 67 kDa type I transmembrane glycoprotein in the immunoglobulin family. CD33, also known as Siglec-3, glycoprotein 67 (gp67), and p67, is expressed on monocytes, activated T cells, myeloid progenitors, granulocytes, dendritic cells, and mast cells. It is absent on erythrocytes, platelets, and lymphoid cells. CD33 binds to sialic acids and acts as a sialic acid-dependent cell adhesion molecule. CD33 also contains intracellular tyrosine-based inhibition motifs (ITIMs), suggesting it functions to inhibit cellular activity.

PRODUCT DETAILS

Clone: WM53

Specificity: CD33

Reactivity: Human

Format: PE-Cyanine5

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine5 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.