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Human peripheral blood lymphocytes were stained with 5 uL (0.125 ug) PE-Cyanine5 Anti-Human CD3 (55-0038) (solid line) or 0.125 ug PE-Cyanine5 Mouse IgG1 isotype control (dashed line).

PE-Cyanine5 Anti-Human CD3 (UCHT1)

SKU 55-0038-T025
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$87.00

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Description

TDS | SDS

The UCHT1 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

The UCHT1 antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of UCHT1 antibody to CD3e can induce cell activation (use format suitable for functional assays). A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3 (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).

UCHT1 antibody reacts with both surface-expressed and intracellular CD3e protein, in contrast to an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. Also, the UCHT1 antibody is reported to be cross-reactive with chimpanzee and has been used for phenotypic analysis of expression by flow cytometry; however the antibody is reported to be unsuitable for induction of T cell activation in this species (Bibollet-Ruche et al. 2009. J. Virol. 82: 10271-10278).

 

Recent Citations:

Konduri V, Joseph SK, Byrd TT, et al. A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma. Sci Transl Med. 2021 May 5;13(592):eabc3196. doi: 10.1126/scitranslmed.abc3196. PMID: 33952672.

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Name PE-Cyanine5 Anti-Human CD3 (UCHT1)
Cat. No. 55-0038
Alternative Names Leu-4, T3
Gene ID 915
Clone UCHT1
Isotype Mouse IgG1, kappa
Reactivity Human
Cross Reactivity Chimpanzee
Format PE-Cyanine5
Application Flow Cytometry
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