Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.

PRODUCT DETAILS

Clone: 2A4

Antibody Type: Monoclonal

Reactivity: Human, Mouse, Rat, All species

Format: PE

Host: Mouse

Isotype: IgG2a, κ

Applications: ICFC - Quality tested

Regulatory Status: RUO

Data Sheets: TDS | SDS

Note to Purchaser: This product is manufactured and supplied by BioLegend, Inc. under an agreement between BioLegend, Inc. and Cytek Biosciences, Inc. Information related to this product is provided by BioLegend, Inc.