The mouse monoclonal antibody clone W6/32 binds to an antigenic determinant shared among HLA-A, HLA-B, and HLA-C human major histocompatibility complex (MHC) class I antigens. MHC class I antigens associated with beta2-microglobulin are expressed by all human nucleated cells and are essential for cell-mediated immune responses and tumor surveillance. W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains among products of the HLA-A, B, and C loci. It immunoprecipitates both 43 kDa and 11-12 kDa chains. The W6/32 antibody can be used to detect and quantify the presence of human Major Histocompatibility Complex (MHC) Class I antigens, specifically HLA-A, HLA-B, and HLA-C, on human cells.

PRODUCT DETAILS

Clone: W6/32

Specificity: MHC Class I (HLA-ABC)

Reactivity: Human

Format: PE

Isotype: Mouse IgG2a, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

For research use only. Not intended for use in diagnostic procedures.