The mouse monoclonal antibody clone AD2 binds to human CD73, a 70 kDa glycosylphosphatidylinositol (GPI)-linked 5’-nucleotidase. CD73, also known as ecto-5’-nucleotidase, has an enzymatic activity to convert extracellular adenosine 5’-monophosphate (AMP) to adenosine. It is present in subsets of both T and B cells and is found at a high level on B cells. It is also expressed in mesenchymal stem cells, follicular dendritic cells, epithelial cells, and endothelial cells. CD73 is also a novel immune checkpoint associated with adenosine metabolism whose expression may promote tumor progression by suppressing antitumor immune response and promoting angiogenesis. CD73 expression level may be a marker of poor cancer prognosis.

PRODUCT DETAILS

Clone: AD2

Specificity: CD73

Reactivity: Human

Format: PE

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Nakamura T, Kubagawa H, et al. 1993. J. Immunol. 151:6933-694
2. Knapp W. et al, 1989. Leucocyte typing IV Oxford University Press. p628-634.

For research use only. Not intended for use in diagnostic procedures.