The mouse monoclonal antibody clone 3F3 binds to human CD72, a 45 kDa type II transmembrane C-type lectin-like receptor expressed predominantly on B cells, from early precursor stages through mature B-cell subsets, as well as on some basophils and mast cells. CD72 modulates B-cell receptor signaling through association with SHP-1 via its ITIM motif, serving as a negative regulatory coreceptor. Clone 3F3 recognizes an extracellular epitope of CD72 and is suitable for identifying B-lineage populations and evaluating maturation or regulatory status in multiparameter flow cytometry panels.

PRODUCT DETAILS

Clone: 3F3

Specificity: CD72

Reactivity: Human

Format: PE

Isotype: Mouse IgG2b

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Kumanogoh A, Watanabe C, Lee I, et al. 2000. Int Immunol. 12:817–824.
2. Bene MC, Nebe T, Bettelheim P, et al. 1995. Leukemia. 9:1783–1786.
3. Orfao A, Schmitz G, Brando B, et al. 1999. Clin Chem Lab Med. 37:983–990.

For research use only. Not intended for use in diagnostic procedures.