The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.

PRODUCT DETAILS

Clone: MEM-59

Specificity: CD43

Reactivity: Human

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Kadaja L, et al. 2004. Oncogene 23:2523-30.
2. Khunkaewla P, et al. 2008. Mol Immunol 45:1703-11.
3. Kadaja-Saarepuu L, et al. 2008. Oncogene 27:1705-15.
4. Mody PD, et al. 2007. Blood 110:2974-82.
5. Stefanová I, et al. 1989. Mol Immunol 26:153-61.
6. Schatzlmaier P, et al. 2015. Sci Signal 8:rs11.
7. Filatov AV, et al. 2007. J Immunol Methods 319:21-33.
8. Cermák L, et al. 2002. J Biol Chem 277:7955-61.
9. Símová S, et al. 2008. Apoptosis 13:423-36.
10. Alvarado M, et al. 1995. Eur J Immunol 25:1051-5.
11. Stefanová I, et al. 1988. Folia Biol (Praha) 34:255-65.
12. Blixt O, et al. 2012. Glycobiology 22:529-42.

For research use only. Not intended for use in diagnostic procedures.