The antibody MEM-M6/1 recognizes an extracellular epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.

PRODUCT DETAILS

Clone: MEM-M6/1

Specificity: CD147

Reactivity: Human

Format: PE

Isotype: Mouse IgG1

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

REFERENCES

1. Kirk P, et al. 2000. EMBO J 19:3896-904.
2. Wilson MC, et al. 2005. J Biol Chem 280:27213-21.
3. Xu D, et al. 2005. Mol Cell Proteomics 4:1061-71.
4. Ruiz S, et al. 2008. J Biol Chem 283:5554-66.
5. Iacono KT, et al. 2007. Exp Mol Pathol 83:283-95.
6. Melchior A, et al. 2008. Exp Cell Res 314:616-28.
7. Schmidt R, et al. 2008. Circ Res 102:302-9.
8. Staffler G, et al. 2003. J Immunol 171:1707-14.
9. Baba M, et al. 2008. Biochem Biophys Res Commun 374:111-6.
10. Cho JY, et al. 2001. Blood 98:374-82.
11. Pistol G, et al. 2007. J Cell Mol Med 11:339-48.
12. Ohradanova-Repic A, et al. 2018. Front Immunol 9:852.
13. Schatzlmaier P, et al. 2015. Sci Signal 8:rs11.
14. Koch C, et al. 1999. Int Immunol 11:777-86.

For research use only. Not intended for use in diagnostic procedures.